FAQ - CRISPR InDel Profiling Platform (CIPP)

CIPP – powered by IDAA, is a sensitive, robust and fast platform for detailed InDel analysis of nuclease treated cells. Optimised with reagents and software, CIPP will offer you a total solution for your next InDel analysis project.

InDel Detection by Amplicon Analysis (IDAA)

• What is IDAA?
• What is the sensitivity of IDAA?
• Does the platform support InDel analysis induced by any nuclease?

DNA Extraction

• What is the most optimal gDNA extraction solution to use?

Tri-primer PCR

• What are the recommendations for IDAA tri-primer design?
• How do I need to purify my primers for IDAA tri-primer PCR?
• What is the optimal size of IDAA tri-primer PCR?
• Can I use any type of PCR enzyme for IDAA tri-primer PCR?
• How to check quality of IDAA tri-primer PCR?

BioInformatics

• ProfileIt - Software program to analyse InDels
• What is included in the standard InDel Data Report?
• Can I get a license to ProfileIt InDel Analysis Software?

Shipment

• Before shipment of IDAA tri-primer PCR samples.
• How much of the IDAA tri-primer PCR sample do I need to ship to COBO?
• What is the most optimal way to ship IDAA tri-primer PCR samples?
• Do I need to add anything to the IDAA tri-primer PCR samples before shipment?
• Can I also ship nuclease treated gDNA or cells to you for InDel analysis?

Applications:

• Applications used with IDAA.
• What is the maximum size of InDels I can detect?
• Will it be possible to detect large deletions using 2 gRNAs?
• Can I detect InDels in Pools?
• Can I quantify large insertions in cells?
• Do COBO also support phenotypic analysis of knockout cells?

Publications

• Please find relevant publications here.