Amplicon Seq
On/Off-Target
- Applications: Identify and quantify InDels, targeted integration
- Sample type: Cells, gDNA or cDNA, PCR from edited and unedited cell lines or primary cells
- Sample prep: Genomic DNA or RNA extraction (cDNA synthesis)
- Library prep/quantification:
- Analysis and bioininformatics:
- Deliverables: File containing sequences and frequencies of observed variants
- Instruments: MiSeq, NextSeq, NovaSeq