Amplicon Seq

On/Off-Target

  • Applications: Identify and quantify InDels, targeted integration
  • Sample type: Cells, gDNA or cDNA, PCR from edited and unedited cell lines or primary cells
  • Sample prep: Genomic DNA or RNA extraction (cDNA synthesis)
  • Library prep/quantification:
  • Analysis and bioininformatics:
  • Deliverables: File containing sequences and frequencies of observed variants
  • Instruments: MiSeq, NextSeq, NovaSeq

 

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