FAQ - CRISPR InDel Profiling Platform (CIPP)

Please find relevant publications below

  • König S, Zhang Y, Wandall HH, Mussolino C, Bennett EP. Fast and Quantitative Identification of Ex Vivo Precise Genome Targeting-Induced Indel Events by IDAA. Methods Mol Biol. 2019, 1961:45-66. doi: 10.1007/978-1-4939-9170-9_
  • Kosicki M, Rajan SS, Lorenzetti FC, Wandall HH, Narimatsu Y, Metzakopian E, Bennett EP. Dynamics of Indel Profiles Induced by Various CRISPR/Cas9 Delivery Methods. 2017, Prog Mol Biol Transl Sci. 2017;152:49-67. doi: 10.1016/bs.pmbts.2017.09.003. Epub 2017 Nov 6.
  • Lonowski LA, Narimatsu Y, Riaz A, Delay CE, Niola F, Duda K, Yang Z, Clausen H, Wandall HH, Hansen SH, Bennett EP and Frödin M,. Genome editing using FACS enrichment of nuclease expressing cells and Indel Detection by Amplicon Analysis (IDAA). 2017, Nat Protoc. Mar;12(3):581-603. doi: 10.1038/nprot.2016.165. Epub 2017 Feb 16.
  • Yang Z, Steentoft C, Hauge C, Hansen L, Thomsen AL, Niola F, Vester-Christensen MB, Frödin M, Clausen H, Wandall HH, Bennett EP. Fast and sensitive detection of indels induced by precise gene targeting. Nucleic Acids Res. 2015 May 19;43(9):e59. doi: 10.1093Lonowski et al.
  • Duda K, Lonowski LA, Kofoed-Nielsen M, Ibarra A, Delay CM, Kang Q, Yang Z, Pruett-Miller SM, Bennett EP, Wandall HH, Davis GD, Hansen SH, Frödin M. High-efficiency genome editing via 2A-coupled co-expression of fluorescent proteins and zinc finger nucleases or CRISPR/Cas9 nickase pairs. Nucleic Acids Res. 2014;42(10). doi: 10.1093.
  • Bennett EP, Petersen BL, Johansen IE, Niu Y, Yang Z, Chamberlain CA, Met Ö, Wandall HH, Frödin M.INDEL detection, the 'Achilles heel' of precise genome editing: a survey of methods for accurate profiling of gene editing induced indels. Nucleic Acids Res. 2020 Dec 2;48(21):11958-11981. doi: 10.1093/nar/gkaa975.

  • Carballar-Lejarazú R, Kelsey A, Pham TB, Bennett EP, James AA. Digital droplet PCR and IDAA for the detection of CRISPR indel edits in the malaria species Anopheles stephensi.Biotechniques. 2020 Feb 10. doi: 10.2144/btn-2019-0103. [Epub ahead of print]

  • Johansen IE, Liu Y, Jørgensen B, Bennett EP, Andreasson E, Nielsen KL, Blennow A, Petersen BL. High efficacy full allelic CRISPR/Cas9 gene editing in tetraploid potato. Sci Rep. 2019 Nov 27;9(1):17715. doi: 10.1038/s41598-019-54126-w

  • Petersen BL, Möller SR, Mravec J, Jørgensen B, Christensen M, Liu Y, Wandall HH, Bennett EP, Yang Z. Improved CRISPR/Cas9 gene editing by fluorescence activated cell sorting of green fluorescence protein tagged protoplasts. BMC Biotechnol. 2019 Jun 17;19(1):36. doi: 10.1186/s12896-019-0530-x.

  • Bennett EP, Ashley M. Jacobi, Garrett R. Rettig and Mark A. Behlke (2018).*GENOME EDITING and ENGINEERING: “From Talens, ZFNs and CRISPRs to Molecular Surgery; Detection of insertion/deletion (indel) events after genome targeting: Pro’s and con’s of the available methods”. (Editor: Krishnarao Appasani) Cambridge University Press, 31st of July, p181-194.

  • Narimatsu Y, Joshi HJ, Zhang Y, Gomes C, Chen YH, Lorenzetti F, Furukawa S, Schjoldager K, Hansen L, Clausen H, Bennett EP, Wandall HH. A validated gRNA library for CRISPR/Cas9 targeting of the human glycosyltransferase genome. Glycobiology. 2018 Jan 5. doi: 10.1093/glycob/cwx101.

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