FAQ - CRISPR InDel Profiling Platform (CIPP)

Please find relevant publications below

  • Eric Paul Bennett, Bent Larsen Petersen, Ida Elisabeth Johansen, Yiyuan Niu, Zhang Yang, Christopher Aled Chamberlain, Özcan Met, Hans H Wandall, Morten Frödin, INDEL detection, the ‘Achilles heel’ of precise genome editing: a survey of methods for accurate profiling of gene editing induced indels, Nucleic Acids Research, Volume 48, Issue 21, 2 December 2020, Pages 11958–11981, https://doi.org/10.1093/nar/gkaa975

 

  • Carballar-Lejarazú R, Kelsey A, Pham TB, Bennett EP, James AA. Digital droplet PCR and IDAA for the detection of CRISPR indel edits in the malaria species Anopheles stephensi.Biotechniques. 2020 Feb 10. doi: 10.2144/btn-2019-0103

 

  • Petersen BL, Möller SR, Mravec J, Jørgensen B, Christensen M, Liu Y, Wandall HH, Bennett EP, Yang Z. Improved CRISPR/Cas9 gene editing by fluorescence activated cell sorting of green fluorescence protein tagged protoplasts. BMC Biotechnol. 2019 Jun 17;19(1):36. doi: 10.1186/s12896-019-0530-x.

 

  • König S, Zhang Y, Wandall HH, Mussolino C, Bennett EP. Fast and Quantitative Identification of Ex Vivo Precise Genome Targeting-Induced Indel Events by IDAA. Methods Mol Biol. 2019, 1961:45-66. doi: 10.1007/978-1-4939-9170-9_4.

 

  • Lonowski LA, Narimatsu Y, Riaz A, Delay CE, Niola F, Duda K, Yang Z, Clausen H, Wandall HH, Hansen SH, Bennett EP and Frödin M,. Genome editing using FACS enrichment of nuclease expressing cells and Indel Detection by Amplicon Analysis (IDAA). 2017, Nat Protoc. Mar;12(3):581-603. doi: 10.1038/nprot.2016.165.

 

  • Yang Z, Steentoft C, Hauge C, Hansen L, Thomsen AL, Niola F, Vester-Christensen MB, Frödin M, Clausen H, Wandall HH, Bennett EP. Fast and sensitive detection of indels induced by precise gene targeting. Nucleic Acids Res. 2015 May 19;43(9):e59. doi: 10.1093
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