CRISPR InDel Profiling Platform (CIPP)
Detect any InDel using the powerful CRISPR InDel Profiling Platform (CIPP™) powered by the IDAA method.
- Do your gene targeting in your cell line of interest using CRISPR (or ZFN, TALEN).
- Ship the genomic DNA (gDNA) to our facility in Denmark (DK). We recommend to use our validated CoboXtract for efficient gDNA extraction from any cell type.
- We do InDel profile analysis on the received gDNA samples and send FSA files for easy InDel profile analysis in ProfileIt (free license available to all users).
- The standard InDel Profile Report will include overview stats, total InDels %, total out-of-frame InDels %, Top5 InDels %, profile image - see example here)
- When shipping gDNA, you will have your results within 10 working days.
- Other formats (pellets and PCR) can also be shipped.
- Quantification of insertions/deletions (InDels) in pools and clones (example here).
- Identification of down to single base InDels.
- Detection of down to 0.1% InDel events.
- Detection of large deletions up to 1000bs.
- Detection small insertions up to 150bs.
- Fast InDel profiling in high-throughput format in pools and clones.
- 96 samples can be analysed in 2 hours.
- Easy detection of bi-allelic targeting events in single cell clones.
- Easy analysis of profiles using the InDel analysis software: ProfileIt.
- Work with any nuclease system (Meganucleases, CRISPR, ZFN, TALEN).
Applications based on customer projects:
- gRNA validation.
- Selection of pools or/and clones with out-of-frame InDels.
- Tag confirmation (up to 150bs).
- Cas9 kinetics.
- Cas9 delivery methods.
- NHEJ inhibitor screening/validation.
- HDR inhibitor screening/validation.
- Drug-resistance allele generation (in-frame insertions/deletions)
- QC of CRISPR modified cells
- Off-target analysis
Why did you choose to use the InDel Profiling Service (IDAA) from COBO Technologies?
"Highly quantitative, minimal amount of material required, detailed profiling of In/dels, easy to interpret the results, highly accessible and excellent customer service, and reasonable prices" VP, Research - NERx BioSciences, US.
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CIPP™, ProfileIt™ and IDAA™ are trademarks of COBO Technologies™. EU/US Patent pending on IDAA™ Method.
CIPP - gRNA Validation (example)
gRNA validation project including 3 different gRNAs targeting hFUT8 gene. InDel profiles were identified and quantified using CIPP and the InDel profiling software ProfileIt 1.0.