Digital PCR (dPCR)
On-Target
- Applications: Absolute quantification of expression, InDel analysis, integrations, translocations
- Sample type: Genomic DNA or RNA/ cDNA from edited and unedited cell lines or primary cells
- Sample prep: Genomic DNA or RNA extraction cDNA synthesis
- Bioinformatics: Statistical analysis comparison to unedited samples Paired t test ( several replicates ), chi squared test for categorical data
