PIPPR Webinar 11th June 2020 - Using SWATH® Acquisition to understand the impact on the Proteome of CRISPR genome editing

Cobo Technologies PIPPR® platform has been specifically designed to understand, at the proteome level, the changes caused by CRISPR genetic editing of cell lines, whether of animal or plant origin.

PIPPR platform includes large-scale proteomics expression analysis using SCIEX SWATH® Acquisition mass spectrometry. SWATH® Acquisition allows the identification and quantification of 3000 to 5000 proteins in any cell line, therefore detecting expression changes when comparing modified vs. wildtype cells. PIPPR® bioinformatics also include the analysis of pathways, gene ontology terms, and protein networks altered by the DNA edition. Since it is a non-targeted approach, not only the expression of the edited gene is monitored, but also the proteins showing differential expression in the same pathways and networks, and even off-target changes, can be detected.

On this webinar, we will show two pilot experiments from Lundbeck, one showing the use of SWATH® Acquisition-based proteomics pilot in assessing CRISPR-based knock-out and second showing the usefulness of SWATH® Acquisition to study full proteome in disease-relevant model systems.

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