Proteomic Expression analysis Platform (PIPPR)

powered by SWATH LC-MS

PIPPR® is the first platform that offer the genome editing scientist an extensive and detailed proteomic expression analysis solution for any type of cells modified with CRISPR-Cas9, Meganucleases, ZFNs or TALENs.

Confirm expected changes and check for un-expected changes of more than 3000 proteins.

PIPPR is powered by SWATH® Acquisition,which gives you label-free proteome scale identification and quantification of potentially any protein in your cell line of interest.

Fast, Robust and Cost-effective combined with an Easy Setup - Just ship your proteins extracted by the proven PIPPR Total Mammalian Protein Extraction Kit from your cells and receive proteomic expression report within 4 weeks.

A detailed bioinformatic solution is also available upon request. For more info, please go to PIPPR Pathway Analysis.

 

Applications:

  • Confirm knockouts of target protein.
  • Compare protein expression between wildtype cells with modified cells.
  • Full proteome expression analysis.
  • Detailed pathway and gene family expression analysis.
  • Quality control of modified cells.
  • Protein expression analysis of potential off-target sites.
  • Treated versus non-treated cells.
  • Base-editors off-target analysis.
  • ...

 

Working with HEK293? Check out the HEK293 PIPPR Signature Library and get details about proteins and pathways included when using the standard PIPPR service.

 

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Interested in InDel mutation analysis ? Include a detailed InDel mutations analysis of your targeted cells using the powerful CRISPR INDEL Profiling Platform, CIPP.

PIPPR® is a registered trademark of COBO Technologies. SWATH®is a registered trademark of SCIEX.

PIPPR - Workflow

PIPPR - Workflow

 

PIPPR Workflow

The PIPPR workflow is very simple and optimised for fast, robust and cost-effective proteomic screenning of CRISPR modified cells.

  1. Do your genome editing in your cell line of interest
  2. Extract total proteins using proven total protein extraction method (example here)
  3. Ship your total protein extracts to COBO.
  4. COBO will do trypsin digestion, SWATH-MS analysis and bioinformatics.
  5. After 2-4 weeks you will receive detailed PIPPR Pathway Analysis Report.

PIPPR News

PIPPR News

CRISPR Researchers to Benefit from COBO Technologies and SCIEX Partnership

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